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Evaluation of a novel dog animal model for peri-implant disease: clinical, radiographic, microbiological and histological assessment.

Authors
  • Martins, Orlando1
  • Ramos, João Carlos2
  • Mota, Marta3, 4
  • Dard, Michel5
  • Viegas, Carlos6
  • Caramelo, Francisco7
  • Nogueira, Célia3, 4
  • Gonçalves, Teresa3, 4
  • Baptista, Isabel Poiares8
  • 1 Institute of Periodontology, Faculty of Medicine, University of Coimbra, 3000-548, Coimbra, Portugal. [email protected] , (Portugal)
  • 2 Institute of Operative Dentistry, Faculty of Medicine, University of Coimbra, 3000-548, Coimbra, Portugal. , (Portugal)
  • 3 Faculty of Medicine, University of Coimbra, 3000-548, Coimbra, Portugal. , (Portugal)
  • 4 Centre for Neuroscience and Cell Biology, University of Coimbra, 3000-548, Coimbra, Portugal. , (Portugal)
  • 5 Department of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University, New York, NY, USA.
  • 6 School of Agrarian and Veterinary Sciences, Department of Veterinary Sciences, Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB, University of Trás-os-Montes e Alto Douro, 5000-801, Vila Real, Portugal. , (Portugal)
  • 7 Laboratory of Biostatistics and Medical Informatics IBILI - Faculty of Medicine, University of Coimbra, 3000-548, Coimbra, Portugal. , (Portugal)
  • 8 Institute of Periodontology, Faculty of Medicine, University of Coimbra, 3000-548, Coimbra, Portugal. , (Portugal)
Type
Published Article
Journal
Clinical Oral Investigations
Publisher
Springer-Verlag
Publication Date
Sep 01, 2020
Volume
24
Issue
9
Pages
3121–3132
Identifiers
DOI: 10.1007/s00784-019-03186-3
PMID: 31916034
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To assess longitudinal peri-implant tissue evaluation in a plaque compromised ligature free dog model, clinically, radiographically, microbiologically and histologically. Six beagle mandibular premolars and first molars were extracted. Plaque accumulated for 16 weeks. Two implants were placed per hemi-mandible. For 17 weeks, control implants (CI) in one hemi-mandible were brushed daily; test implants (TI) in the other were not. These parameters were then assessed: clinically, probing depth (PD), bleeding-on-probing (BOP), presence of plaque (PP) and clinical attachment level (CAL); radiographically, marginal bone level; microbiologically, counts for Streptococcus spp., Fusobacterium spp., Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia and total bacterial load. At week 17, histomorphometric analysis was performed (MM-ISH (mucosal margin-implant shoulder); ISH-fBIC (implant shoulder-first bone-to-implant contact); MM-aJE (mucosal margin-apical area junctional epithelium); MM-aINF (mucosal margin-apical limit of the inflammatory infiltrate); %INF (percentage of inflammatory infiltrate)). At week 17, TI had significant increased PD, BOP, PP and CAL versus baseline. All clinical variables presented intergroup differences. There was no intergroup difference for radiographic bone loss (p > 0.05). Total bacteria, Fusobacterium spp., A. actinomycetemcomitans and P. gingivalis had intergroup differences. There was no statistically significant intergroup difference for ISH-fBIC. Longitudinal microbiology evaluation detected a shift period. Final intergroup microbiological differences were the basis of W17 clinical intergroup differences, with higher values in TI. Microbiological and clinical changes detected in peri-implant tissues were compatible with onset of peri-implant disease. Despite histological inflammatory intergroup difference, no histological or radiographic intergroup bone loss was detected. This study set-up describes a valuable method for generating "true" early peri-implant defects without mechanical trauma.

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