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Evaluation of inhibitor-combination mCIM for detecting MBL-producing Enterobacterales using three MBL inhibitors.

Authors
  • Yamada, Kageto1, 2
  • Sasaki, Masakazu2, 1
  • Imai, Waka1
  • Murakami, Hinako1
  • Morita, Toshisuke3, 1
  • Aoki, Kotaro2
  • Ishii, Yoshikazu2
  • Tateda, Kazuhiro2
  • 1 Department of Clinical Laboratory, Toho University Medical Centre Omori Hospital, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan. , (Japan)
  • 2 Department of Microbiology and Infectious Disease, Toho University Graduate School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan. , (Japan)
  • 3 Department of Laboratory Medicine, Toho University Graduate School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan. , (Japan)
Type
Published Article
Journal
Journal of Medical Microbiology
Publisher
Microbiology Society
Publication Date
Nov 01, 2019
Volume
68
Issue
11
Pages
1604–1606
Identifiers
DOI: 10.1099/jmm.0.001073
PMID: 31513006
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The increase in carbapenemase-producing Enterobacterales (CPE), including metallo-β-lactamase (MBL) producers, is a severe global health concern. Thus, highly sensitive and specific methods for detecting MBL producers are needed. In this study, we tested the detectability of MBL-producing Enterobacterales against three types of MBL inhibitors (sodium mercaptoacetate, SMA; ethylenediaminetetraacetic acid, EDTA; and dipicolinic acid, DPA) used in combination with a modified carbapenem inactivation method (mCIM). These inhibitor-combination mCIMs were tested against 129 CPE (IMP, 93; NDM, 11; KPC, 13; NMC, 1; OXA-48, 11) and 75 non-CPE. For evaluation of MBL inhibitors, we used two concentrations for each of the three inhibitors: DPA (200 and 300 mg l- 1), EDTA (5 and 10 mM), and SMA (1500 and 3000 mg l- 1). The overall sensitivities of SMA, EDTA and DPA were 97.1-99.0 %, 81.7-99.0 % and 88.5-96.2 %, respectively. Moreover, each method showed high specificity (99.0-100 %). Although inhibitor-combination mCIMs were highly sensitive and specific for the detection of MBL producers, we found that sensitivity was dependent on the concentration of inhibitors.

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