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Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo

Authors
  • Zhao, Pengfei1
  • Wang, Chaofei1
  • Ding, Jun2
  • Zhao, Chengfeng3
  • Xia, Yingjun1
  • Hu, Yanli1
  • Zhang, Li1
  • Zhou, Yanqin1
  • Zhao, Junlong1
  • Fang, Rui1
  • 1 Huazhong Agricultural University,
  • 2 Animal Disease Prevention and Control Center, Jingshan, 431800 Hubei China
  • 3 Animal Disease Prevention and Control Center, Anlu, 432600 Hubei China
Type
Published Article
Journal
Parasitology Research
Publisher
Springer-Verlag
Publication Date
Mar 10, 2021
Pages
1–11
Identifiers
DOI: 10.1007/s00436-021-07105-y
PMID: 33689009
PMCID: PMC7943400
Source
PubMed Central
Keywords
Disciplines
  • Protozoology - Original Paper
License
Unknown

Abstract

Coccidiosis triggered by Eimeria tenella is accompanied by haemorrhagic caecum and high morbidity. Vaccines are preferable choices to replace chemical drugs against coccidiosis. Surface antigens of apicomplexan parasites can adhere to host cells during the infection process. Therefore, truncated fragments coding E. tenella surface antigen 16 (EtSAG16) and 22 (EtSAG22) were cloned into pET-28a prokaryotic vector to express recombinant protein 16 (rEtSAG16) and 22 (rEtSAG22), respectively. Likewise, pEGFP-N1-EtSAG16 and pEGFP-N1-EtSAG22 plasmids were constructed using pEGFP-N1 eukaryotic vector. Further, pEGFP-N1-EtSAG4-16-22 multiple gene plasmid carrying EtSAG4, 16 and 22 were designed as cocktail vaccines to study integral immunoprotective effects. Western blot and RT-PCR (reverse transcription) assay were performed to verify expressions of EtSAG16 and 22 genes. Immunoprotective effects of recombinant protein or DNA vaccine were evaluated using different doses (50 or 100 μg) in vivo. All chickens in the vaccination group showed higher cytokine concentration (IFN-γ and IL-17), raised IgY antibody level, increased weight gain, lower caecum lesion score and reduced oocyst shedding compared with infection control groups ( p < 0.05). The highest anticoccidial index (ACI) value 173.11 was from the pEGFP-N1-EtSAG4-16-22 plasmid (50 μg) group. In conclusion, EtSAG16 and 22 might be alternative candidate genes for generating vaccines against E. tenella infection.

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