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Evaluation of heparin immobilized chitosan-PEG microbeads for charcoal encapsulation and endotoxin removal.

Authors
Type
Published Article
Journal
Artificial cells, blood substitutes, and immobilization biotechnology
Publication Date
Volume
28
Issue
1
Pages
65–77
Identifiers
PMID: 10676578
Source
Medline

Abstract

A technique is described to encapsulate activated charcoal for hemoperfusion to be used in an artificial liver support. Activated charcoal was encapsulated within chitosan-PEG matrix and subsequently surface modified with PGE1 or heparin (hep-AC-PEGCB) via the glutaraldehyde functionalities. This novel matrix was used as the supports for perfusion of endotoxin, under a flow rate of 30 ml/mt. Endotoxin adsorption was quantitatively measured by the method of Limulus Amebocyte lysate test. It seems, the hep-AC-PEGCB may be a good adsorbent system for the removal of toxic endotoxin, and the system may be useful for detoxification of blood. The hep-AC-PEGCB matrix had improved biocompatibility as demonstrated from their hemolytic potential and charcoal release. However, further studies are needed to determine their behaviour under clinical conditions.

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