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Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry.

Authors
  • Barbosa, Joana
  • Rodrigues, Acácio Gonçalves
  • Pérez, Maria José
  • Pina-Vaz, Cidália
Type
Published Article
Journal
Memórias do Instituto Oswaldo Cruz
Publisher
SciELO
Publication Date
Dec 01, 2014
Volume
109
Issue
8
Pages
1078–1080
Identifiers
DOI: 10.1590/0074-0276140267
PMID: 25424449
Source
Medline
License
Unknown

Abstract

Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.

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