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Evaluation of freeze-dried human sera as a novel approach for ATR-FTIR spectroscopic analysis as compared to conventionally used thin dry film sera.

Authors
  • Ali, Salmann1
  • Naseer, Khulla1
  • Hussain, Syed Zajif2
  • Qazi, Javaria3
  • 1 Faculty of Biological Sciences, Qauid-I-Azam University, Islamabad, Pakistan. , (Pakistan)
  • 2 Department of Chemistry & Chemical Engineering, Syed Babar Ali School of Science and Engineering, Lahore University of Management Sciences (LUMS), DHA, Lahore Cantt, 54792, Pakistan. , (Pakistan)
  • 3 Faculty of Biological Sciences, Qauid-I-Azam University, Islamabad, Pakistan. [email protected] , (Pakistan)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Dec 01, 2019
Volume
41
Issue
12
Pages
1355–1360
Identifiers
DOI: 10.1007/s10529-019-02739-6
PMID: 31628577
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Evaluation of the diagnostic potential of freeze dried sera in comparison to thin dry film analysis for recording ATR-FTIR spectra. For this purpose, we compared our novel sample preparation technique i.e. freeze dried with conventional technique i.e. thin dry film sera. Using both methods ATR-FTIR spectra were recorded from Salmonella Typhi infected and healthy control human sera samples. When PCA was applied PC1 scores showed more inter-class variation among infected and healthy controls when freeze dried sample was used (90 %) as compared to thin dry film method (46 %). Potential of ATR-FTIR for discrimination of bio-molecules between two classes of samples is enhanced when freeze dried sera instead of thin dry film method is used.

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