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Evaluation of the expression of collagen type I in porous calcium phosphate ceramics implanted in an extra-osseous site.

Authors
  • Qu, S X
  • Guo, X
  • Weng, J
  • Cheng, J C Y
  • Feng, B
  • Yeung, H Y
  • Zhang, X D
Type
Published Article
Journal
Biomaterials
Publisher
Elsevier
Publication Date
Feb 01, 2004
Volume
25
Issue
4
Pages
659–667
Identifiers
PMID: 14607504
Source
Medline
License
Unknown

Abstract

The aim of the present study is to demonstrate the newly formed tissue in calcium phosphate (Ca/P) ceramics after extra-osseous implantation by histological and immunohistochemistry (IHC) methods. Synthesis porous Ca/P ceramics without adding any growth factor and living cell were implanted in the dorsal muscle of dogs for 1 and 2 months. Undecalcified and decalcified sections were stained by hematoxylin and eosin (H&E), and IHC, respectively. The histological results showed the beginning of osteogenesis and angiogenesis after being implanted for 1 month and the obvious new bone formation after being implanted for 2 months. IHC were conducted via the avidin-biotin peroxidase complex (ABC) method and the primary antibody was collagen type I. IHC results indicated that collagen type I was expressed within osteoblast-like cells and newly formed bone-like tissue in Ca/P ceramics after 1 month, and in the mineralized matrix of newly formed bone and osteoblasts, some osteocytes and some lacunae after 2 months. No cartilage and chondrocytes were observed in the histological and IHC-stained sections. Evidence of intramembranous osteogenesis was confirmed.

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