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Evaluation of equilibrium gel filtration chromatography for the study of protein binding of aluminum in normal and uremic sera.

Authors
  • Bertholf, R L
  • Wills, M R
  • Savory, J
Type
Published Article
Journal
Clinical physiology and biochemistry
Publication Date
Jan 01, 1985
Volume
3
Issue
5
Pages
271–276
Identifiers
PMID: 4053493
Source
Medline
License
Unknown

Abstract

Normal and uremic serum samples were chromatographed on a gel filtration column and fractions were analyzed for protein and aluminum content. Whereas much of the aluminum in the serum samples eluted with protein, a significant fraction appeared to be associated with low molecular weight species. Analysis of column fractions for aluminum binding by a competitive binding assay indicated no aluminum-binding serum constituents in these later eluting fractions. Uremic serum appeared, however, to contain aluminum-binding constituents of a lower molecular weight than in normal serum.

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