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Evaluation of Elafin Immunohistochemical Expression as Marker of Cervical Cancer Severity

Authors
  • Longatto-Filho, Adhemar
  • Fregnani, José Humberto
  • Mafra da Costa, Allini
  • de Araujo-Souza, Patricia Savio
  • Scapulatempo-Neto, Cristovam
  • Herbster, Suellen
  • Boccardo, Enrique
  • Termini, Lara
Type
Published Article
Journal
Acta Cytologica
Publisher
S. Karger AG
Publication Date
Dec 03, 2020
Volume
65
Issue
2
Pages
165–174
Identifiers
DOI: 10.1159/000512010
PMID: 33271565
Source
Karger
Keywords
License
Green
External links

Abstract

Introduction: The main risk factor for the development of cervical cancer (CC) is persistent infection by human papillomavirus (HPV) oncogenic types. In order to persist, HPV exhibits a plethora of immune evasion mechanisms. PI3/Elafin (Peptidase Inhibitor 3) is an endogenous serine protease inhibitor involved in epithelial protection against pathogens. PI3/Elafin’s role in CC is still poorly understood. Materials and Methods: In the present study, we addressed PI3/Elafin protein detection in 123 CC samples by immunohistochemistry and mRNA expression in several datasets available at Gene Expression Omnibus and The Cancer Genome Atlas platforms. Results: We observed that PI3/Elafin is consistently downregulated in CC samples when compared to normal tissue. Most of PI3/Elafin-positive samples exhibited this protein at the plasma membrane. Besides, high PI3/Elafin expression at the cellular membrane was more frequent in in situ stages I + II than in invasive cervical tumor stages III + IV. This indicates that PI3/Elafin expression is gradually lost during the CC progression. Of note, advanced stages of CC were more frequently associated with a more intense PI3/Elafin reaction in the nuclei and cytoplasm. Conclusion: Our results suggest that PI3/Elafin levels and subcellular localization may be used as a biomarker for CC severity.

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