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Evaluation of effectiveness of microwave irradiation for disinfection of silicone elastomeric impression material.

Authors
  • Bhasin, Abhilasha1
  • Vinod, V2
  • Bhasin, Vinny3
  • Mathew, Xavier4
  • Sajjan, Suresh5
  • Ahmed, Syed Tauqheer2
  • 1 Department of Prosthodontics, Hitkarini Dental College and Hospital, 153 Adarsh Nagar, Narmada Road, Jabalpur, 482002 Madhya Pradesh India. , (India)
  • 2 Department of Prosthodontics, The Oxford Dental College and Hospital, Bangalore, Karnataka India. , (India)
  • 3 Department of Orthodontics, Mansarovar Dental College, Bhopal, Madhya Pradesh India. , (India)
  • 4 Department of Prosthodontics, College of Dental Sciences, Davangere, Karnataka India. , (India)
  • 5 Vishnu Dental College, Bhimavaram, Andra Pradesh India. , (India)
Type
Published Article
Journal
Journal of Indian Prosthodontic Society
Publication Date
Jun 01, 2013
Volume
13
Issue
2
Pages
89–94
Identifiers
DOI: 10.1007/s13191-012-0230-x
PMID: 24431716
Source
Medline
Keywords
License
Unknown

Abstract

Use of domestic microwave oven has been suggested as a method of disinfecting a number of dental materials used in dental practice. This study was done to analyse the effect of microwave irradiation on vinyl polysiloxane putty impression material (3M ESPE, Express™ STD) contaminated with test organisms (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans. 180 square shaped specimens of addition silicon putty material were prepared and divided into 3 groups for three test organisms. The 3 groups were subdivided into 4 subgroups (n = 15) for different exposure parameters (control group 5, 6 and 7 min exposure at 650 W. The specimens were contaminated using standard inoculums of test organism and then were irradiated using domestic microwaves. Broth cultures of the control and test group specimens were plated on selective media culture plates. Colonies formed were counted. Data analyses included Kruskal-Walli's ANOVA and Mann-Whitney's tests. Nil values shows complete elimination of C. albicans and P. aeruginosa after 5, 6 and 7 min exposure. Staphylococcus aureus showed colonies with the mean value of 7.6 × 10(3) ± 2.3 × 10(3), 4.6 × 10(3) ± 2.6 × 10(3) after 5 and 6 min respectively and nil values after 7 min exposure. 5 min exposure caused complete elimination of C. albicans and P. aeruginosa strains, while 7 min exposure eliminated S. aureus completely.

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