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Evaluation of the effect of storage at -70 degrees C for six months on hemostatic function testing in dogs.

Authors
  • Bateman, S W
  • Mathews, K A
  • Abrams-Ogg, A C
  • Lumsden, J H
  • Johnstone, I B
Type
Published Article
Journal
Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire
Publication Date
Jul 01, 1999
Volume
63
Issue
3
Pages
216–220
Identifiers
PMID: 10480465
Source
Medline
License
Unknown

Abstract

Freezing is a routine method of storage for plasma that is to be used in evaluating certain aspects of hemostatic function in many species. The purpose of this study was to evaluate the effect of storage at -70 degrees C for 6 mo on canine plasma samples. On fresh and frozen plasma from 12 clinically healthy dogs, prothrombin time, activated partial thromboplastin time, thrombin clotting time, fibrinogen determination, antithrombin III activity, fragment D and E assay, and protamine sulfate test were performed. Clinical agreement analysis was utilized to determine the effect of such storage on all assays. Individual differences detected between fresh and frozen samples were all within 2 standard deviations of the mean difference. With the exception of the activated partial thromboplastin time, storing canine plasma at -70 degrees C for 6 mo has no significant effect on hemostatic function, as assessed by these tests.

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