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Evaluation and diagnostic usefulness of domestic and imported enzyme-linked immunosorbent assays for detection of human immunodeficiency virus type 1 antibody in India.

Authors
  • Iqbal, H Syed1
  • Solomon, Suniti
  • Murugavel, K G
  • Solomon, Sunil Suhas
  • Balakrishnan, P
  • 1 VHS-YRG Centre for AIDS Research and Education, Voluntary Health Services Campus, Taramani, Chennai 600 113, India. , (India)
Type
Published Article
Journal
Clinical and Diagnostic Laboratory Immunology
Publisher
American Society for Microbiology
Publication Date
Dec 01, 2005
Volume
12
Issue
12
Pages
1425–1428
Identifiers
PMID: 16339066
Source
Medline
Language
English
License
Unknown

Abstract

Diagnosis of human immunodeficiency virus (HIV) infection is important for patient management and prevention of new infections. The number of test kits available for the detection of HIV antibodies is unprecedented. In order to identify appropriate test kits, we evaluated a variety of commercial kits manufactured abroad as well as in India. The plasma and serum specimens (n=264) were collected from individuals attending the Voluntary Counseling and Testing Centre at the YRG Centre for AIDS and Education. The specimens were used to evaluate six commercially available HIV test kits: Enzaids HIV 1+2, HIV-CheX, Murex HIV-1.2.0, Genscreen HIV 1/2 version 2, Vironostika HIV Uni-Form II Ag/Ab, and CombAids RS Advantage. High sensitivities and specificities (>or=99%) were observed for the Enzaids, Murex, Vironostika, and CombAids assays. HIV-CheX showed the highest number of false-positive and false-negative results. The Genscreen test also gave many false positives. The study indicated that the Enzaids, Murex, and Vironostika enzyme-linked immunosorbent assay kits and the CombAids RS Advantage rapid assay could be used to achieve acceptable results for the detection of HIV antibodies. A combination of two tests is recommended to optimize the efficiency of HIV antibody testing algorithms, especially when evaluation with an HIV Western blot confirmatory test is not possible.

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