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Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin.

Authors
  • Robert, Jacques1
  • Edholm, Eva-Stina2, 3
  • De Jesus Andino, Francisco2
  • 1 Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA. [email protected]
  • 2 Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
  • 3 The Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway. , (Norway)
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2018
Volume
1865
Pages
265–273
Identifiers
DOI: 10.1007/978-1-4939-8784-9_19
PMID: 30151773
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Analyses by flow cytometry and differential counts by cytospin of peripheral blood leukocytes are two reliable and convenient methods used to assess the immune status and immune responses in the amphibian Xenopus. Here, we describe these methods and discuss their challenges and limitations.

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