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Estimation of kinetic cell-cycle-related gene expression in G1 and G2 phases from immunofluorescence flow cytometry data.

Authors
  • Jacobberger, J W
  • Sramkoski, R M
  • Wormsley, S B
  • Bolton, W E
Type
Published Article
Journal
Cytometry
Publication Date
Mar 01, 1999
Volume
35
Issue
3
Pages
284–289
Identifiers
PMID: 10082310
Source
Medline
License
Unknown

Abstract

By this method, the upper and lower bounds of cyclin B1 expression could be estimated and kinetic expression through G1, G2, and M modeled. Together with direct measurements in S phase, expression of B1 throughout the entire cell cycle of DU 145 cells could be modeled. The method should be generally applicable given model-specific assumptions.

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