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Estimation of fluorescence probing depth dependence on the distance between source and detector using Monte Carlo modeling

Authors
  • Ong, Yi Hong
  • Zhu, Timothy C.
Type
Published Article
Journal
Proceedings of SPIE--the International Society for Optical Engineering
Publication Date
Mar 12, 2021
Volume
11628
Identifiers
DOI: 10.1117/12.2582818
PMID: 34083858
PMCID: PMC8171224
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

Photosensitizer fluorescence emission during photodynamic therapy (PDT) can be used to estimate for in vivo photosensitizer concentration. We built a surface contact probe with 405nm excitation light source to obtain Photofrin fluorescence signal during clinical PDT. The probe was equipped with multiple detector fibers that were located at distances between 0.14 to 0.87 cm laterally from the excitation source fiber. In this study, we investigated the probing depth of fluorescence in biological tissue with different source-detector separation using our contact probe setup. We used Monte Carlo method to simulate the 405nm excitation light and 630nm fluorescence probing depth at various source and detector (SD) separations. The results provided insight to the most probable depth of origin of detected fluorescence at each SD separation and help to understand the in vivo depth distribution of clinically measured Photofrin concentration.

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