The Esterification of Chlorophyllide a in Greening Bean Leaves
- Authors
- Type
- Published Article
- Journal
- Zeitschrift für Naturforschung C
- Publisher
- Walter de Gruyter GmbH
- Publication Date
- Oct 01, 1978
- Volume
- 33
- Issue
- 9-10
- Pages
- 712–714
- Identifiers
- DOI: 10.1515/znc-1978-9-1018
- Source
- De Gruyter
- Keywords
- License
- Green
Abstract
The accumulation of esterfied chlorophyll a (Chl a) in etiolated bean leaves was determined during a dark period of 60 minutes after 1 minute irradiation with white light. The separation of the pigments esterified with geranylgeraniol (GG), dihydrogeranylgeraniol (DHGG), tetrahydrogeranylgeraniol (THGG) and phytol (P) was performed by HPLC. The last step for chlorophyll a biosynthesis is confirmed to be an esterification of chlorophyllide a to ChlGG followed by a stepwise hydrogenation of the pigment-bounded alcohols to ChlDHGG and ChlTHGG and finally to Chl a.
