Affordable Access

deepdyve-link
Publisher Website

Establishment of microcosm biofilm models that reproduce a cariogenic diet intake.

Authors
  • Florez Salamanca, Elkin Jahir1
  • Dantas, Rayssa Mariana1
  • Rodriguez, Martha Juliana2
  • Klein, Marlise Inêz1
  • 1 Department of Dental Materials and Prosthodontics, São Paulo State University (Unesp), School of Dentistry, Araraquara, São Paulo, Brazil. , (Brazil)
  • 2 School of Dentistry, University Santo Tomas, Bucaramanga, Colombia. , (Colombia)
Type
Published Article
Journal
Biofouling
Publisher
Informa UK (Taylor & Francis)
Publication Date
Nov 01, 2020
Volume
36
Issue
10
Pages
1196–1209
Identifiers
DOI: 10.1080/08927014.2020.1862093
PMID: 33349045
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Biofilms were developed from human saliva on bovine enamel discs in four experimental conditions to investigate dental caries development: feast and famine (M1), abundance and scarcity (M2), three meals daily (M3), and three meals plus two snacks daily (M4). The main difference between these models was the diet for microbial growth. The evaluations included verifying the pH of the spent culture media and analyzing the enamel discs for demineralization (microhardness and roughness) and biofilms (biomass, viable populations of mutans streptococci, and total microbiota). Two major behaviors were observed: M1 and M2 promoted an acidic environment, while M3 and M4 maintained pH values closer to neutral. The demineralization process was slower in the neutral groups but more pronounced in M3, while a greater increase in microbiota and biomass was observed over time for both neutral groups. Thus, the M3 model was better at mimicking the oral environment that leads to demineralization.

Report this publication

Statistics

Seen <100 times