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Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene

  • Lee, Hyunah
  • Nam, Donggyu
  • Choi, Jae Kyung
  • Araúzo‐Bravo, Marcos J
  • Kwon, Soon-Yong
  • Zaehres, Holm
  • Lee, Taehee
  • Park, Chan Young
  • Kang, Hyun Wook
  • shoeler, hans robert
  • Kim, Jeong Beom
Publication Date
Feb 15, 2016
[email protected]
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The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cell-enriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC. / open

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