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[Establishment of cytokine-independent human myelodysplastic cell line and its characteristics].

Authors
  • Shao, Ze-Ye
  • Chen, Bao-An
  • Xia, Guo-Hua
  • Xue, Meng
  • Gao, Chong
  • Ding, Jia-Hua
  • Sun, Yun-Yu
  • Wang, Jun
  • Cheng, Jian
  • Zhao, Gang
  • Gao, Xue-Zhi
Type
Published Article
Journal
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology
Publication Date
Apr 01, 2005
Volume
13
Issue
2
Pages
298–303
Identifiers
PMID: 15854296
Source
Medline
License
Unknown

Abstract

This study was aimed to establish a cytokine-independent human myelodysplastic cells line from bone marrow of a patient with MDS-CMML. This cell line was incubated in mixed culture of RPMI 1640 and DMEM with 15% bovine serum, but without cytokines; its biological characteristics were identified by morphology, surface marker profiles, cell proliferation, differentiation and apoptosis. The results showed that the established cell line could not depend on cytokines for long-term survival and growth, and could differentiate into colony-forming unit-macrophage, colony-forming unit-megakaryocyte. In conclusion, a cytokine-independent human myelodysplastic syndrome cell line, named MDS-JSN04 (MDS Nanjing Jiansu 04), was established. Its partial biological characteristics were identified and clarified.

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