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Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping.

Authors
  • Guerrero, Brenda I1
  • Guerra, Ma Engracia2
  • Rodrigo, Javier3
  • 1 Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA); (CITA-Universidad de Zaragoza), Instituto Agroalimentario de Aragón - IA2; [email protected]
  • 2 Departamento de Hortofruticultura, Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto de Investigaciones Agrarias Finca La Orden.
  • 3 Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA); (CITA-Universidad de Zaragoza), Instituto Agroalimentario de Aragón - IA2.
Type
Published Article
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Publication Date
Nov 09, 2020
Issue
165
Identifiers
DOI: 10.3791/61897
PMID: 33226025
Source
Medline
Language
English
License
Unknown

Abstract

The Japanese plum cultivars commonly grown are interspecific hybrids derived from crosses between the original Prunus salicina with other Prunus species. Most hybrids exhibit gametophytic self-incompatibility, which is controlled by a single and highly polymorphic S-locus that contains multiple alleles. Most cultivated hybrids are self-incompatible and need pollen from a compatible donor to fertilize their flowers. Establishing pollination requirements in Japanese plum is becoming increasingly important due to the high number of new cultivars with unknown pollination requirements. In this work, a methodology for the determination of pollination requirements in Japanese plum-type hybrids is described. Self-(in)compatibility is determined by hand-pollinations in both the field and in the laboratory, followed by monitoring pollen tube elongation with fluorescence microscopy, and also monitoring fruit maturation in the field. Selection of pollinizer cultivars is assessed by combining the identification of S-genotypes by PCR analysis with the monitoring of flowering time in the field. Knowing the pollination requirements of cultivars facilitates the selection of cultivars for the design of new orchards and allows the early detection of productivity problems related with pollination deficiency in established orchards.

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