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The essential role of mRNA degradation in understanding and engineering E. coli metabolism.

Authors
  • Roux, Charlotte1
  • Etienne, Thibault A2
  • Hajnsdorf, Eliane3
  • Ropers, Delphine4
  • Carpousis, A J5
  • Cocaign-Bousquet, Muriel6
  • Girbal, Laurence7
  • 1 TBI, Université de Toulouse, CNRS, INRAE, INSA, 31077 Toulouse, France; UMR8261, CNRS, Université de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France. Electronic address: [email protected] , (France)
  • 2 TBI, Université de Toulouse, CNRS, INRAE, INSA, 31077 Toulouse, France; Univ. Grenoble Alpes, Inria, 38000 Grenoble, France. Electronic address: [email protected] , (France)
  • 3 UMR8261, CNRS, Université de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France. Electronic address: [email protected] , (France)
  • 4 Univ. Grenoble Alpes, Inria, 38000 Grenoble, France. Electronic address: [email protected] , (France)
  • 5 TBI, Université de Toulouse, CNRS, INRAE, INSA, 31077 Toulouse, France; LMGM, Université de Toulouse, CNRS, UPS, CBI, 31062 Toulouse, France. Electronic address: [email protected] , (France)
  • 6 TBI, Université de Toulouse, CNRS, INRAE, INSA, 31077 Toulouse, France. Electronic address: [email protected] , (France)
  • 7 TBI, Université de Toulouse, CNRS, INRAE, INSA, 31077 Toulouse, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Biotechnology advances
Publisher
Oxford : Pergamon Press
Publication Date
Jul 21, 2021
Pages
107805–107805
Identifiers
DOI: 10.1016/j.biotechadv.2021.107805
PMID: 34302931
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Metabolic engineering strategies are crucial for the development of bacterial cell factories with improved performance. Until now, optimal metabolic networks have been designed based on systems biology approaches integrating large-scale data on the steady-state concentrations of mRNA, protein and metabolites, sometimes with dynamic data on fluxes, but rarely with any information on mRNA degradation. In this review, we compile growing evidence that mRNA degradation is a key regulatory level in E. coli that metabolic engineering strategies should take into account. We first discuss how mRNA degradation interacts with transcription and translation, two other gene expression processes, to balance transcription regulation and remove poorly translated mRNAs. The many reciprocal interactions between mRNA degradation and metabolism are also highlighted: metabolic activity can be controlled by changes in mRNA degradation and in return, the activity of the mRNA degradation machinery is controlled by metabolic factors. The mathematical models of the crosstalk between mRNA degradation dynamics and other cellular processes are presented and discussed with a view towards novel mRNA degradation-based metabolic engineering strategies. We show finally that mRNA degradation-based strategies have already successfully been applied to improve heterologous protein synthesis. Overall, this review underlines how important mRNA degradation is in regulating E. coli metabolism and identifies mRNA degradation as a key target for innovative metabolic engineering strategies in biotechnology. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

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