Affordable Access

Escherichia coli membrane proteins with an affinity for deoxyribonucleic acid.

Authors
  • M Kohiyama
  • R Kollek
  • W Goebel
  • A Kepes
Publication Date
Feb 01, 1977
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

From the membrane fraction of Escherichia coli K-12 strain, four protein fractions (peaks I, IIa, IIb, and III) which have affinity for deoxyribonucleic acid (DNA) have been isolated. The molecular weights of these proteins are between 12,000 and 8,000. Only the peak III fraction contains a protein that binds preferentially to single-stranded DNA, whereas the others contain proteins that bind also to double-stranded DNA. The binding activity of the peak IIb protein is inhibited in the presence of polyuridylic acid. Peak I and peak IIa protein fractions behave like hydrophobic proteins.

Report this publication

Statistics

Seen <100 times