Affordable Access

Escherichia coli kgtP encodes an alpha-ketoglutarate transporter.

Authors
  • Seol, W
  • Shatkin, A J
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
May 01, 1991
Volume
88
Issue
9
Pages
3802–3806
Identifiers
PMID: 2053984
Source
Medline
License
Unknown

Abstract

The witA gene located between pss and rrnG on the Escherichia coli chromosome encodes a 432-amino acid protein. It is homologous to a human hepatoma glucose transporter and to E. coli membrane proteins that transport citrate (CitA), arabinose (AraE), and xylose (XylE), and, like these carrier proteins, WitA also contains 12 highly hydrophobic putative membrane-spanning regions. Gene disruption mutants constructed in two E. coli strains grew slowly or not at all, depending on genetic background, in M9 minimal medium containing alpha-ketoglutarate. Growth on alpha-ketoglutarate and uptake of alpha-[14C]ketoglutarate were restored by transformation with plasmids containing witA. These complementation studies indicate that WitA is an alpha-ketoglutarate transporter and should be renamed kgtP(alpha-ketoglutarate permease).

Report this publication

Statistics

Seen <100 times