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Escherichia coli expression vectors containing a protein kinase recognition motif, His6-tag and hemagglutinin epitope.

Authors
  • Kelman, Z
  • Yao, N
  • O'Donnell, M
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Dec 01, 1995
Volume
166
Issue
1
Pages
177–178
Identifiers
PMID: 8529886
Source
Medline
License
Unknown

Abstract

Escherichia coli expression vectors, based on the pET system, were constructed to allow fusion of a protein kinase (PK) recognition motif, a hemagglutinin (HG) epitope-tag and a His6-tag at the N-terminal portion of a protein of interest. The fusion proteins, that result from expression using these vectors, can be phosphorylated in vitro using cAMP-dependent PK, immunoprecipitated using monoclonal antibody against the HG-epitope, and can be rapidly purified using a Ni2+ column.

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