Affordable Access

Escherichia coli expression vectors containing a protein kinase recognition motif, His6-tag and hemagglutinin epitope.

Authors
Type
Published Article
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
166
Issue
1
Pages
177–178
Identifiers
PMID: 8529886
Source
Medline

Abstract

Escherichia coli expression vectors, based on the pET system, were constructed to allow fusion of a protein kinase (PK) recognition motif, a hemagglutinin (HG) epitope-tag and a His6-tag at the N-terminal portion of a protein of interest. The fusion proteins, that result from expression using these vectors, can be phosphorylated in vitro using cAMP-dependent PK, immunoprecipitated using monoclonal antibody against the HG-epitope, and can be rapidly purified using a Ni2+ column.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments