Affordable Access

deepdyve-link
Publisher Website

Escargot restricts niche cell to stem cell conversion in the Drosophila testis.

Authors
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Volume
7
Issue
3
Pages
722–734
Identifiers
DOI: 10.1016/j.celrep.2014.04.025
Source
Hunter Lab
License
Unknown

Abstract

Stem cells reside within specialized microenvironments, or niches, that control many aspects of stem cell behavior. Somatic hub cells in the Drosophila testis regulate the behavior of cyst stem cells (CySCs) and germline stem cells (GSCs) and are a primary component of the testis stem cell niche. The shutoff (shof) mutation, characterized by premature loss of GSCs and CySCs, was mapped to a locus encoding the evolutionarily conserved transcription factor Escargot (Esg). Hub cells depleted of Esg acquire CySC characteristics and differentiate as cyst cells, resulting in complete loss of hub cells and eventually CySCs and GSCs, similar to the shof mutant phenotype. We identified Esg-interacting proteins and demonstrate an interaction between Esg and the corepressor C-terminal binding protein (CtBP), which was also required for maintenance of hub cell fate. Our results indicate that niche cells can acquire stem cell properties upon removal of a single transcription factor in vivo.

Report this publication

Statistics

Seen <100 times