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Error analysis in titration microcalorimetry of biochemical systems.

Authors
  • Vickers, L P
  • Hopkins, H P Jr
  • Ali, S Z
  • Carey, V
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Mar 01, 1985
Volume
145
Issue
2
Pages
257–265
Identifiers
PMID: 3893212
Source
Medline
License
Unknown

Abstract

A simplified method for titrations of biochemical systems is described as well as extensive error propagation through the data analysis. This work uses a Tronac Model 450 isoperibol titration calorimeter. Sample volumes of 2 ml are used and total heats of less than 5 mcal can be routinely measured. The binding of 3'-CMP to bovine pancreatic ribonuclease A is used to illustrate the methods. The binding enthalpy can be determined with a standard deviation of 1.5% and the free energy with a standard deviation of 2 to 3%.

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