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Equine infectious anemia: preparation of a liquid antigen extract for the agar-gel immunodiffusion and complement-fixation tests.

Authors
  • Boulanger, P
  • Bannister, G L
  • Carrier, S P
Type
Published Article
Journal
Canadian journal of comparative medicine : Revue canadienne de médecine comparée
Publication Date
Apr 01, 1972
Volume
36
Issue
2
Pages
116–123
Identifiers
PMID: 4259924
Source
Medline
License
Unknown

Abstract

An agar-gel immunodiffusion test recommended for the diagnosis of equine infectious anemia was evaluated. Our preliminary observations confirmed those of Coggins concerning the mechanism of the test and the results obtained. Furthermore, emphasis was put on the difficulties encountered in the production of spleen antigens with an optimum amount of reactivity. Acetone-ether extraction procedures for the preparation of a liquid antigen extract are described. This type of antigen was reactive in the complement-fixation test in 1:8 or greater dilution and it is proposed to use the complement-fixation test in assessing and standardizing the liquid antigen extract activity to be used in the immunodiffusion test. This antigen can also be concentrated or diluted, if required, to meet the reactivity of a standard antigen used in the test.

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