Affordable Access

deepdyve-link
Publisher Website

EPAS1 Gain-of-Function Mutation Contributes to High-Altitude Adaptation in Tibetan Horses

Authors
  • Liu, Xuexue1, 2
  • Zhang, Yanli1, 2
  • Li, Yefang1, 2
  • Pan, Jianfei1, 2
  • Wang, Dandan1, 2
  • Chen, Weihuang3
  • Zheng, Zhuqing3
  • He, Xiaohong1, 2
  • Zhao, Qianjun1, 2
  • Pu, Yabin1, 2
  • Guan, Weijun1, 2
  • Han, Jianlin2, 4
  • Orlando, Ludovic5, 6
  • Ma, Yuehui1, 2
  • Jiang, Lin1, 2
  • 1 Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, PR China
  • 2 CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, PR China
  • 3 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China
  • 4 International Livestock Research Institute (ILRI), Nairobi, Kenya
  • 5 Lundbeck Foundation GeoGenetics Center, University of Copenhagen, Denmark
  • 6 Laboratoire d'Anthropobiologie Moléculaire et d'Imagerie de Synthèse, CNRS, UMR 5288, Université Paul Sabatier (UPS), Toulouse, France
Type
Published Article
Journal
Molecular Biology and Evolution
Publisher
Oxford University Press
Publication Date
Jul 04, 2019
Volume
36
Issue
11
Pages
2591–2603
Identifiers
DOI: 10.1093/molbev/msz158
PMID: 31273382
PMCID: PMC6805228
Source
PubMed Central
Keywords
License
Unknown

Abstract

High altitude represents some of the most extreme environments worldwide. The genetic changes underlying adaptation to such environments have been recently identified in multiple animals but remain unknown in horses. Here, we sequence the complete genome of 138 domestic horses encompassing a whole altitudinal range across China to uncover the genetic basis for adaptation to high-altitude hypoxia. Our genome data set includes 65 lowland animals across ten Chinese native breeds, 61 horses living at least 3,300 m above sea level across seven locations along Qinghai-Tibetan Plateau, as well as 7 Thoroughbred and 5 Przewalski’s horses added for comparison. We find that Tibetan horses do not descend from Przewalski’s horses but were most likely introduced from a distinct horse lineage, following the emergence of pastoral nomadism in Northwestern China ∼3,700 years ago. We identify that the endothelial PAS domain protein 1 gene ( EPAS1 , also HIF2A ) shows the strongest signature for positive selection in the Tibetan horse genome. Two missense mutations at this locus appear strongly associated with blood physiological parameters facilitating blood circulation as well as oxygen transportation and consumption in hypoxic conditions. Functional validation through protein mutagenesis shows that these mutations increase EPAS1 stability and its hetero dimerization affinity to ARNT (HIF1B). Our study demonstrates that missense mutations in the EPAS1 gene provided key evolutionary molecular adaptation to Tibetan horses living in high-altitude hypoxic environments. It reveals possible targets for genomic selection programs aimed at increasing hypoxia tolerance in livestock and provides a textbook example of evolutionary convergence across independent mammal lineages.

Report this publication

Statistics

Seen <100 times