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Eosinophil Granulocyte and NK Cell-Mediated Platelet Destruction in Immune Thrombocytopenia

Authors
  • Wang, Shuguo1
  • Liu, Fenghai1
  • Zhao, Hongguo2
  • 1 The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao, China , Qingdao (China)
  • 2 The Affiliated Hospital of Qingdao University, Qingdao, China , Qingdao (China)
Type
Published Article
Journal
Indian Journal of Hematology and Blood Transfusion
Publisher
Springer India
Publication Date
May 17, 2017
Volume
34
Issue
1
Pages
121–124
Identifiers
DOI: 10.1007/s12288-017-0830-z
Source
Springer Nature
Keywords
License
Yellow

Abstract

To investigate the contribution and mechanism of eosinophil granulocytes and NK cells-mediated cytotoxicity to the pathogenesis of ITP. Mononuclear cells and platelets were prepared from the bone marrow of 16 ITP patients and 10 healthy controls. Separately, eosinophil granulocytes and NK cells were selected with magnetic microbeads. As the target cells, the autologous platelets were cultured with eosinophil granulocytes and NK cells respectively for 6 h and then stained with annexin V. Ratio of platelets expressing annexin V was determined by flow cytometry. The fraction of NK cells expressing perforin, granzyme B, FasL and TNF were determined by flow cytometry. Human eosinophil cationic protein (ECP) level in the bone marrow was determined by ECP ELISA kit. The annexin V positive platelet ratio of the ITP group was significantly higher than that of the control group; the expression rates of granzyme B, perforin, FasL on NK cells of the ITP group were significantly higher than those of the control group. Expression of human ECP in bone marrow of immune thrombocytopenia patients was higher than that of healthy controls. NK cells and eosinophil granulocytes are activated in ITP and might be involved in the pathogenesis of ITP.

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