Affordable Access

deepdyve-link
Publisher Website

Enzyme-linked immunosorbent assay for detection of serum or mucosal isotype-specific IgG and IgA whole-virus antibody to influenza A virus in swine.

Authors
  • Gauger, Phillip C
  • Loving, Crystal L
  • Vincent, Amy L
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2014
Volume
1161
Pages
303–312
Identifiers
DOI: 10.1007/978-1-4939-0758-8_25
PMID: 24899439
Source
Medline
License
Unknown

Abstract

Enzyme-linked immunosorbent assays can be used to detect isotype-specific anti-influenza antibodies in biological samples to characterize the porcine immune response to influenza A virus. The isotype antibody assay is based on an indirect ELISA using whole influenza virus as antigen and detection antibodies directed against porcine IgG and IgA. Samples such as serum, nasal wash, and bronchoalveolar lavage fluid allow for evaluation of systemic, upper, and lower respiratory tract mucosal antibody responses, respectively. The isotype ELISA assay is performed in a 96-well format using anti-swine detection antibodies conjugated to an enzyme that catalyze a color change reaction. The optical density of the sample is measured using an automated plate reader. The assay is useful to characterize the IgG or the IgA response to challenge or vaccination against specific influenza virus isolates in different compartments of the immune system.

Report this publication

Statistics

Seen <100 times