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Enzyme immunoassays for bromocriptine and its metabolites.

Authors
  • Valente, D
  • Ezan, E
  • Créminon, C
  • Delaforge, M
  • Benech, H
  • Pradelles, P
  • Grognet, J M
Type
Published Article
Journal
Journal of immunoassay
Publication Date
Nov 01, 1996
Volume
17
Issue
4
Pages
297–320
Identifiers
PMID: 8957762
Source
Medline
License
Unknown

Abstract

We have developed two bromocriptine enzyme immunoassays with different specificities for applications in human and animal pharmacokinetic studies. The first assay uses antibodies directed against the cyclopeptide structure of bromocriptine, and is specific for untransformed bromocriptine. The second assay uses antibodies directed against the bromolysergic part of the molecule and allows the measurement of both bromocriptine and its metabolites. Enzymatic tracers were obtained by covalent coupling of bromocriptine analogs to acetylcholinesterase from the electric eel Electrophorus electricus. Both assays have a limit of detection of 10 pg/ml and a limit of quantification of 50 pg/ml. The specificity of the assays was determined following fractionation by high-performance liquid chromatography of rat samples obtained after administration of bromocriptine.

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