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Enzyme immunoassay of H-Y antigen: experimental and clinical applications.

Authors
  • 1
  • 1 Center for Reproductive Biology, Collierville, TN 38017.
Type
Published Article
Journal
Differentiation; research in biological diversity
Publication Date
Volume
35
Issue
2
Pages
122–125
Identifiers
PMID: 3443229
Source
Medline
License
Unknown

Abstract

A newly developed enzyme-linked immunosorbent assay (ELISA) was applied for studying H-Y antigen in buffaloes, cattle, horses and humans. A monoclonal H-Y antibody was absorbed with cells from males or females and was then tested against fluid samples known to contain soluble H-Y antigen. In this system, positive absorption manifested itself by a fall in optical density relative to the optical density scored using unabsorbed antibody; this finding signified the presence of H-Y in the absorbing cells. In each of the four species, the fall in optical density was pronounced after absorption with male cells, but some decrease was also evident after absorption with female cells, indicating a degree of nonspecific attachment of the antibody.

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