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Enzyme-immunoassay in the detection of hepatitis B surface antigen.

Authors
Type
Published Article
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
15
Issue
4
Pages
343–353
Identifiers
PMID: 406327
Source
Medline
License
Unknown

Abstract

A new enzyme-immunoassay (EIA, Hepanostika Microelisa System) for the detection of hepatitis B surface antigen was evaluated against other methods, namely complement fixation, Hepanosticon, AusRIA II and Finnish Red Cross Radioimmunoassay (FRC-RIA). EIA detected the greatest number of positive samples in a serum panel consisting of 142 sera from clinical hepatitis patients. FRC-RIA was the most sensitive method for subtype ad, while EIA detected the ay specimen at the highest dilution. None of the test systems gave the 'optimal' result in the screening test, and it is proposed that a separate procedure for each antigen subtype should be carried out to detect the greatest number of positive samples.

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