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Enzyme Assay in Microsomes Below Zero Degrees

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PMC
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  • Biology
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Abstract

Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0° were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0°.

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