Affordable Access

Access to the full text

Enzymatic synthesis of glutathione using engineered Saccharomyces cerevisiae

Authors
  • Chen, Jia-li1
  • Xie, Liang1
  • Cai, Jing-jing1
  • Yang, Cheng-shuai1
  • Duan, Xue-hui1
  • 1 Nanchang University, State Key Laboratory of Food Science and Technology, School of Life Science and Food Engineering, Nanchang, 330047, People’s Republic of China , Nanchang (China)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Mar 30, 2013
Volume
35
Issue
8
Pages
1259–1264
Identifiers
DOI: 10.1007/s10529-013-1191-9
Source
Springer Nature
Keywords
License
Yellow

Abstract

Two single gene cassettes, each containing one of the individual gene (γ-glutamylcysteine synthetase gene GSH1 or glutathione synthetase gene GSH2), were constructed under the control of alcohol dehydrogenase (ADH1) promoter and their respective native terminators. The recombinant plasmids constructed with Kanr or Hygr as the selective markers and were transformed into Saccharomyces cerevisiae separately and jointly. Three engineered strains, GSH1-enhanced strain S.TS013/GSH1, GSH2-enhanced strain S.TS013/GSH2 and GSH1+GSH2 double-enhanced strain S.TS013/GSH1+GSH2, were constructed. Glutathione production using the recombinant strains to improve was then determined. By the cell dosage proportions of two engineered strains (S.TS013/GSH1, S.TS013/GSH2) and a two-stage reaction, GSH productivity increased by 84 and 59 % over that of the host strain and the S.TS013/GSH1+GSH2 strain, respectively.

Report this publication

Statistics

Seen <100 times