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Enzymatic synthesis of delta sleep-inducing peptide.

Authors
  • Sakina, K
  • Kawazura, K
  • Morihara, K
Type
Published Article
Journal
International journal of peptide & protein research
Publisher
Wiley (Blackwell Publishing)
Publication Date
Feb 01, 1988
Volume
31
Issue
2
Pages
245–252
Identifiers
PMID: 3366553
Source
Medline
License
Unknown

Abstract

The delta sleep-inducing peptide was assembled enzymatically from three tripeptide fragments. All the peptide bonds were prepared by either papain- or alpha-chymotrypsin-mediated synthesis. Secondary hydrolysis was suppressed by introducing N alpha-protected amino acid or peptide esters as carboxyl components and using an alkaline pH. The protected nonapeptide was oxidized with ferric chloride to deprotect the C-terminal phenylhydrazide and then hydrogenated. The homogeneous peptide was obtained by reversed phase high-performance liquid chromatography. Comparison of enzymatic and chemical preparations showed no obvious differences.

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