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Enzymatic synthesis of adenosine 5'-O-(3-[35S]-thiotriphosphate).

Authors
Type
Published Article
Journal
Biochimica et Biophysica Acta
0006-3002
Publisher
Elsevier
Publication Date
Volume
483
Issue
1
Pages
1–5
Identifiers
PMID: 880303
Source
Medline
License
Unknown

Abstract

A method for the enzymatic synthesis of adenosine 5'-O-(3-[35S]thiotriphosphate) is described. The method involves the thiophosphate exchange of adenosine 5'-O-(3-thiotriphosphate) with [35S]thiophosphate with the aid of glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase in the presence of 3-phosphoglycerate. The method is also applicable for the synthesis of guanosine 5'-O-(3-[35S]thiotriphosphate).

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