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Enzymatic kinetic of cellulose hydrolysis: inhibition by ethanol and cellobiose.

Authors
  • Bezerra, Rui M F
  • Dias, Albino A
Type
Published Article
Journal
Applied biochemistry and biotechnology
Publication Date
Jul 01, 2005
Volume
126
Issue
1
Pages
49–59
Identifiers
PMID: 16014998
Source
Medline
License
Unknown

Abstract

The ethanol effect on the Trichoderma reesei cellulases was studied to quantify and clarify this inhibition type. To determine inhibition parameters of crude cellulase and purified exoglucanase Cel7A, integrated Michaelis-Menten equations were used assuming the presence of two inhibitors: cellobiose as the reaction product and ethanol as a possible bioproduct of cellulose fermentation. It was found that hydrolysis of cellulose by crude enzyme follows a model that considers noncompetitive inhibition by ethanol, whereas Cel7A is very slightly competitively inhibited. Crude cellulase is much more inhibited (K(iul) = K(icl) = 151.9 mM) than exoglucanase Cel7A (K(icl) = 1.6 x 1015 mM). Also, calculated inhibition constants showed that cellobiose inhibition is more potent than ethanol inhibition both for the crude enzyme as well as exoglucanase Cel7A.

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