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Enzymatic degradation of deoxynivalenol by a novel bacterium, Pelagibacterium halotolerans ANSP101.

Authors
  • Zhang, Jing1
  • Qin, Xiaojuan1
  • Guo, Yongpeng1
  • Zhang, Qiongqiong1
  • Ma, Qiugang1
  • Ji, Cheng1
  • Zhao, Lihong2
  • 1 State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, PR China. , (China)
  • 2 State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, PR China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
Publication Date
Mar 19, 2020
Volume
140
Pages
111276–111276
Identifiers
DOI: 10.1016/j.fct.2020.111276
PMID: 32199966
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Deoxynivalenol (DON), a toxic secondary metabolite produced by Fusarium species that mainly infests cereals such as wheat and corn, threatens human and livestock health. The present study describes the characterization of a novel bacterial strain, Pelagibacterium halotolerans ANSP101 which is capable of transforming DON to less-toxic product 3-keto-deoxynivalenol by the oxidation of the C3 hydroxyl group. Strain ANSP101 was isolated from a seawater sample from a depth of 55 m in Chinese Bohai sea. The strain was identified as Pelagibacterium halotolerans by morphology characterization and 16S rDNA gene sequencing. The DON degrading activity of strain ANSP101 was predominantly attributed to the bacterial cell lysate. Besides, the cell lysate was sensitive to sodium dodecyl sulfate, heat, and proteinase K treatment, indicating that the intracellular proteins or enzymes are responsible for the DON degradation. The optimal temperature and pH for the maximal degradation of DON were 40 °C and pH 8.0 by the cell lysate. These results provide the potential use of P. halotolerans ANSP101 as a detoxification agent for DON decontamination in cereals and feed. Copyright © 2020 Elsevier Ltd. All rights reserved.

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