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Enterocloster alcoholdehydrogenati sp. nov., a Novel Bacterial Species Isolated from the Feces of a Patient with Alcoholism.

Authors
  • Oikawa, Daiki1, 2
  • Fukui, Kohei1
  • Aoki, Yuichi3, 4
  • Waki, Toshiyuki1
  • Takahashi, Seiji1
  • Shimoyama, Takefumi5
  • Nakayama, Toru6
  • 1 Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8579, Japan. , (Japan)
  • 2 Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Oiwake-Cho, Kitashirakawa, Sakyo-Ku, Kyoto, 606-8502, Japan. , (Japan)
  • 3 Department of Integrative Genomics, Tohoku Medical Megabank Organization, Seiryo 2-1, Sendai, Miyagi, 980-8573, Japan. , (Japan)
  • 4 Graduate School of Information Sciences, Tohoku University, Aoba 6-3-09, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8679, Japan. , (Japan)
  • 5 Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8579, Japan. [email protected]. , (Japan)
  • 6 Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8579, Japan. [email protected]. , (Japan)
Type
Published Article
Journal
Current microbiology
Publication Date
Apr 19, 2023
Volume
80
Issue
5
Pages
187–187
Identifiers
DOI: 10.1007/s00284-023-03285-1
PMID: 37074467
Source
Medline
Language
English
License
Unknown

Abstract

Strain C5-48T, an anaerobic intestinal bacterium that potentially accumulates acetaldehyde at levels exceeding its minimum mutagenic concentration (50 µM) in the colon and rectum, was isolated from the feces of a patient with alcoholism. The 16S rRNA gene sequence of strain C5-48T showed high similarity to the corresponding sequences of Lachnoclostridium edouardi Marseille-P3397T (95.7%) and Clostridium fessum SNUG30386T (94.7%). However, phylogenetic analysis using the sequences of the 16S rRNA, rpoB, and hsp60 genes and whole-genome analysis strongly suggested that C5-48T should be included in the genus Enterocloster. The novelty of strain C5-48T was further confirmed by comprehensive average nucleotide identity (ANI) calculations based on its whole-genome sequence, which showed appreciable ANI values with known Enterocloster species (e.g., 74.3% and 73.4% with Enterocloster bolteae WAL 16351T and Enterocloster clostridioformis ATCC 25537T, respectively). The temperature range for growth of strain C5-48T was 15-37 °C with an optimum of 37 °C. The pH range for growth was 5.5-10.5 with an optimum of 7.5. The major constituents of the cell membrane lipids of strain C5-48T were 16:0, 14:0, and 18:1 ω7c dimethyl acetal fatty acids. On the basis of the genotypic and phenotypic properties, Enterocloster alcoholdehydrogenati sp. nov. is proposed, with the type strain C5-48T (= JCM 33305T = DSM 109474T). © 2023. The Author(s).

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