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Enhancing Neurological Competence of Nanoencapsulated Cordyceps/Turmeric Extracts in Human Neuroblastoma SH-SY5Y Cells

Authors
  • Kaokaen, Palakorn
  • Sorraksa, Natchadaporn
  • Phonchai, Ruchee
  • Chaicharoenaudomrung, Nipha
  • Kunhorm, Phongsakorn
  • Noisa, Parinya
Type
Published Article
Journal
Cellular and Molecular Bioengineering
Publisher
Springer-Verlag
Publication Date
Dec 13, 2022
Volume
16
Issue
1
Pages
81–93
Identifiers
DOI: 10.1007/s12195-022-00752-w
PMID: 36660588
PMCID: PMC9842810
Source
PubMed Central
Keywords
Disciplines
  • Original Article
License
Unknown

Abstract

Introduction Neurological diseases, including Alzheimer's, Parkinson's diseases, and brain cancers, are reportedly caused by genetic aberration and cellular malfunction. Herbs with bioactive compounds that have anti-oxidant effects such as cordyceps and turmeric, are of interest to clinical applications due to their minimal adverse effects. The aim of study is to develop the nanoencapsulated cordyceps and turmeric extracts and investigate their capability to enhance the biological activity and improve neuronal function. Methods Human neuroblastoma SH-SY5Y cells were utilized as a neuronal model to investigate the properties of nanoencapsulated cordyceps or turmeric extracts, called CMP and TEP, respectively. SH-SY5Y cells were treated with either CMP or TEP and examined the biological consequences, including neuronal maturation and neuronal function. Results The results showed that both CMP and TEP improved cellular uptake efficiency within 6 h by 2.3 and 2.8 times, respectively. Besides, they were able to inhibit cellular proliferation of SH-SY5Y cells up to 153- and 218-fold changes, and increase the expression of mature neuronal markers ( TUJ1 , PAX6 , and NESTIN ). Upon the treatment of CMP and TEP, the expression of dopaminergic-specific genes ( LMX1B , FOXA2 , EN1 , and NURR1 ), and the secretion level of dopamine were significantly improved up to 3.3-fold and 3.0-fold, respectively, while the expression of Alzheimer genes ( PSEN1 , PSEN2 , and APP ), and the secretion of amyloid precursor protein were significantly reduced by 32-fold and 108-fold, respectively. Importantly, the autophagy activity was upregulated by CMP and TEP at 6.3- and 5.5-fold changes, respectively. Conclusions This finding suggested that the nanoencapsulated cordyceps and turmeric extracts accelerated neuronal maturation and alleviated neuronal pathology in human neural cells. This paves the way for nanotechnology-driven drug delivery systems that could potentially be used as an alternative medicine in the future for neurological diseases.

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