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Enhancer Analyses Using Chicken Embryo Electroporation.

Authors
  • Uchikawa, Masanori1
  • Nishimura, Naoko2
  • Iwafuchi-Doi, Makiko3
  • Kondoh, Hisato4
  • 1 Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan. , (Japan)
  • 2 Shizuoka Prefecture Special Education Schools, Yahatano, Ito, Shizuoka, 413-0232, Japan. , (Japan)
  • 3 Smilow Center for Translational Research, University of Pennsylvania Perelman School of Medicine, 3400 Civic Center Blvd., Bldg. 421, Philadelphia, PA, 19104-5157, USA.
  • 4 Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto, 603-8555, Japan. [email protected] , (Japan)
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2017
Volume
1650
Pages
191–202
Identifiers
DOI: 10.1007/978-1-4939-7216-6_12
PMID: 28809022
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Chicken embryo electroporation is a powerful tool used to identify and analyze enhancers involved in developmental gene regulation. In this chapter, the basic procedures and underlying principles of enhancer analysis using chicken embryo electroporation are described in the following steps: (1) identification of enhancers in a wide genomic region, (2) determination of the full enhancer region, (3) definition of the core enhancer regions, and (4) analysis of a functional transcription factor binding sequences in the core region.

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