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Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG.

Authors
  • Zhang, Pingping
  • Zhou, Wei
  • Wang, Peng
  • Wang, Li
  • Tang, Mingli
Type
Published Article
Journal
Brazilian Journal of Microbiology
Publisher
Elsevier
Publication Date
Jan 01, 2013
Volume
44
Issue
1
Pages
189–195
Identifiers
DOI: 10.1590/S1517-83822013005000017
PMID: 24159305
Source
Medline
Keywords
License
Unknown

Abstract

Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 °C. The alginate beads had well stability, retained 85% ability of enzyme production even after 7 cycles of repeated batch fermentation. These results showed the immobilization technique was a feasible and economical method for chitosansase production by Gongronella sp. JG.

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