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Enhancement of Bone Marrow-Derived Mesenchymal Stem Cell Osteogenesis and New Bone Formation in Rats by Obtusilactone A.

Authors
  • Lin, Yi-Hsiung1, 2, 3
  • Chen, Chung-Yi4
  • Chou, Liang-Yin5, 6, 7
  • Chen, Chung-Hwan8, 9, 10, 11
  • Kang, Lin12
  • Wang, Chau-Zen13, 14, 15, 16
  • 1 Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 2 Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 3 Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 4 School of Medical and Health Sciences, Fooyin University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 5 Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 6 Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 7 Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 8 Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 9 Department of Orthopaedics, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 10 Department of Orthopedics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 11 Division of Adult Reconstruction Surgery, Department of Orthopedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 12 Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. [email protected] , (Taiwan)
  • 13 Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 14 Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 15 Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
  • 16 Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. [email protected] , (Taiwan)
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Nov 15, 2017
Volume
18
Issue
11
Identifiers
DOI: 10.3390/ijms18112422
PMID: 29140298
Source
Medline
Keywords
License
Unknown

Abstract

The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias. Micro-CT images and H&E staining showed more trabecular bone at the needle-implanted site in the OA group than the normal saline group. Thus, OA confers an osteoinductive effect on BMSCs via induction of osteogenic marker gene expression, such as BMP2 and Runx2 expression and subsequently elevates ALP activity and mineralization, followed by enhanced trabecular bone formation in rat tibias. Therefore, OA is a potential osteoinductive drug to stimulate new bone formation by BMSCs.

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