Affordable Access

deepdyve-link
Publisher Website

Enhancement of antibody production against rabies virus by uridine 5′-triphosphate in mice

Authors
  • Iwaki, Yoshimi
  • Sakai, Yusuke
  • Ochiai, Kenji
  • Umemura, Takashi
  • Sunden, Yuji1, 2, 3, 4
  • 1 Laboratory of Comparative Pathology
  • 2 Department of Veterinary Clinical Sciences
  • 3 School of Veterinary Medicine
  • 4 Hokkaido University
Type
Published Article
Journal
Microbes and Infection
Publisher
Elsevier
Publication Date
Jan 01, 2013
Accepted Date
Nov 22, 2013
Volume
16
Issue
3
Pages
196–202
Identifiers
DOI: 10.1016/j.micinf.2013.11.012
Source
Elsevier
Keywords
License
Unknown

Abstract

Extracellular nucleotides such as adenosine 5′-triphospate (ATP) and uridine 5′-triphosphate (UTP) interact with P2 purinergic receptors on the surface of phagocytic cells and induce various physiological reactions. In this study, the production of antibody in mice immunized with an inactivated rabies vaccine containing these nucleotides was investigated. Injection of inactivated rabies vaccine with UTP, but not with ATP, induced significantly higher serum antibody production in mice. The enhancement of antibody production by UTP was inhibited by an anti-P2Y4 receptor antibody. In an air pouch experiment, UTP treatment increased the number of monocytes and macrophages infiltrating the pouch and up-regulated the gene expression of IL-4 and IL-13 in the regional lymph nodes. These results suggested that UTP admixed with rabies vaccine activates Th2 cells and induces a humoral immune response. Furthermore, the survival rate of mice immunized with a rabies vaccine admixed with UTP before rabies virus challenge was slightly higher than that of control mice. In conclusion, UTP can act as a vaccine adjuvant to enhance antibody production against the rabies virus in mice.

Report this publication

Statistics

Seen <100 times