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Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in repeated batch culture with optimized substitution of culture medium

Authors
  • Jin, Il-Hyuck1
  • Jung, Dae-Young2
  • Son, Chang-Woo2
  • Kim, Sung-Koo3
  • Gao, Wa4
  • Chung, Chung-Han4
  • Lee, Jin-Woo1, 4
  • 1 Dong-A University, Department of Biotechnology, Busan, 604-714, Korea , Busan (South Korea)
  • 2 Korea Bio-Solution Co., Busan, 612-020, Korea , Busan (South Korea)
  • 3 Pukyung National University, Department of Biotechnology, Busan, 608-737, Korea , Busan (South Korea)
  • 4 BK21 Bio-Silver Program of Dong-A University, Busan, 604-714, Korea , Busan (South Korea)
Type
Published Article
Journal
Biotechnology and Bioprocess Engineering
Publisher
Springer-Verlag
Publication Date
Apr 20, 2011
Volume
16
Issue
2
Pages
245–255
Identifiers
DOI: 10.1007/s12257-010-0120-1
Source
Springer Nature
Keywords
License
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Abstract

In this study, a process of removing a half volume of culture broth and replacing it with an equal volume of substituted solution was developed to enhance the production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica HS-2001. The optimal substitution time and volume of the substituted solution were found to be 48 h after cultivation and 50% of the initial volume of the culture broth. The optimal composition of the substituted solution was determined to be 20.0 g/L glucose, 10.0 g/L soybean pomace, 0.1 g/L MgSO4·7H2O, 0.9 g/L NH4NO3, and 5.0 g/L potassium phosphate, which was the same composition as the medium developed in a previous study for the production of PS-7 by B. indica HS-2001. The total amount and productivity of PS-7 by B. indica HS-2001 with a substitution under optimized conditions in a 7 L bioreactor for 96 h were 49.28 g and 0.51 g/h, respectively, which were 1.76 and 1.31-foldgreater values than those without a substitution for 72 h.

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