Affordable Access

deepdyve-link
Publisher Website

Enhanced healing of diabetic wounds by topical administration of adipose tissue-derived stromal cells overexpressing stromal-derived factor-1: biodistribution and engraftment analysis by bioluminescent imaging.

Authors
  • Di Rocco, Giuliana1
  • Gentile, Antonietta
  • Antonini, Annalisa
  • Ceradini, Francesca
  • Wu, Joseph C
  • Capogrossi, Maurizio C
  • Toietta, Gabriele
  • 1 Laboratorio di Biologia Vascolare e Medicina Rigenerativa, Centro Cardiologico Fondazione Monzino - IRCCS, Via Parea 4, 20138 Milan, Italy. , (Italy)
Type
Published Article
Journal
Stem Cells International
Publisher
Hindawi Publishing Corporation (Sage-Hindawi Access to Research)
Publication Date
Dec 26, 2010
Volume
2011
Pages
304562–304562
Identifiers
DOI: 10.4061/2011/304562
PMID: 21234108
Source
Medline
Language
English
License
Unknown

Abstract

Chronic ulcers represent a major health problem in diabetic patients resulting in pain and discomfort. Conventional therapy does not guarantee adequate wound repair. In diabetes, impaired healing is partly due to poor endothelial progenitor cells mobilisation and homing, with altered levels of the chemokine stromal-derived factor-1 (SDF-1) at the wound site. Adipose tissue-associated stromal cells (AT-SCs) can provide an accessible source of progenitor cells secreting proangiogenic factors and differentiating into endothelial-like cells. We demonstrated that topical administration of AT-SCs genetically modified ex vivo to overexpress SDF-1, promotes wound healing into diabetic mice. In particular, by in vivo bioluminescent imaging analysis, we monitored biodistribution and survival after transplantation of luciferase-expressing cells. In conclusion, this study indicates the therapeutic potential of AT-SCs administration in wound healing, through cell differentiation, enhanced cellular recruitment at the wound site, and paracrine effects associated with local growth-factors production.

Report this publication

Statistics

Seen <100 times