Affordable Access

deepdyve-link
Publisher Website

Enhanced activity and enantioselectivity of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1 by acetone treatment.

Authors
  • Cong, Fangdi
  • Xing, Kezhi
  • Gao, Renjun
  • Cao, Shugui
  • Zhang, Guirong
Type
Published Article
Journal
Applied Biochemistry and Biotechnology
Publisher
Springer-Verlag
Publication Date
Oct 01, 2011
Volume
165
Issue
3-4
Pages
795–801
Identifiers
DOI: 10.1007/s12010-011-9297-9
PMID: 21643808
Source
Medline
License
Unknown

Abstract

To improve the activity and enantioselectivity of hyperthermophilic archaeon Aeropyrum pernix K1 esterase (APE1547) and its mutants, they were purified by acetone-treated method. It was found that the acetone treatment not only caused APE1547 and its mutants to display higher activity and enantioselectivity but also saved more than 90% of time spent in purifying them by Ni-chelating column. In hydrolysis of p-nitrophenyl caprylate, the acetone-treated APE1547 and mutant A containing the following substitutions R11G, L36P, V225A, I551L, and A564T showed 5.7- and 6.9-fold active increase, respectively. In the resolution of 2-octanol acetate, the acetone-treated mutant A had a 9-fold enantioselective increase relative to that purified by Ni-chelating column. In addition, the impact of pH, temperature, and chemical reagents on activity of APE1547 and mutant A was discussed in this paper.

Report this publication

Statistics

Seen <100 times