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Engineering meiotic recombination pathways in rice.

Authors
  • Fayos, Ian1, 2
  • Mieulet, Delphine1, 2
  • Petit, Julie1, 2
  • Meunier, Anne Cécile1, 2
  • Périn, Christophe1, 2
  • Nicolas, Alain3, 4
  • Guiderdoni, Emmanuel1, 2
  • 1 Cirad, UMR AGAP, Montpellier, France. , (France)
  • 2 Université de Montpellier, Cirad-Inra-Montpellier SupAgro, Montpellier, France. , (France)
  • 3 Institut Curie, CNRS UMR 3244, University PSL, Paris, France. , (France)
  • 4 Meiogenix, Paris, France. , (France)
Type
Published Article
Journal
Plant biotechnology journal
Publication Date
Nov 01, 2019
Volume
17
Issue
11
Pages
2062–2077
Identifiers
DOI: 10.1111/pbi.13189
PMID: 31199561
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In the last 15 years, outstanding progress has been made in understanding the function of meiotic genes in the model dicot and monocot plants Arabidopsis and rice (Oryza sativa L.), respectively. This knowledge allowed to modulate meiotic recombination in Arabidopsis and, more recently, in rice. For instance, the overall frequency of crossovers (COs) has been stimulated 2.3- and 3.2-fold through the inactivation of the rice FANCM and RECQ4 DNA helicases, respectively, two genes involved in the repair of DNA double-strand breaks (DSBs) as noncrossovers (NCOs) of the Class II crossover pathway. Differently, the programmed induction of DSBs and COs at desired sites is currently explored by guiding the SPO11-1 topoisomerase-like transesterase, initiating meiotic recombination in all eukaryotes, to specific target regions of the rice genome. Furthermore, the inactivation of 3 meiosis-specific genes, namely PAIR1, OsREC8 and OsOSD1, in the Mitosis instead of Meiosis (MiMe) mutant turned rice meiosis into mitosis, thereby abolishing recombination and achieving the first component of apomixis, apomeiosis. The successful translation of Arabidopsis results into a crop further allowed the implementation of two breakthrough strategies that triggered parthenogenesis from the MiMe unreduced clonal egg cell and completed the second component of diplosporous apomixis. Here, we review the most recent advances in and future prospects of the manipulation of meiotic recombination in rice and potentially other major crops, all essential for global food security. © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

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