Affordable Access

Access to the full text

Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins

Authors
  • Kim, Kangsan1, 2
  • Choe, Donghui1, 2
  • Lee, Dae-Hee
  • Cho, Byung-Kwan1, 2, 3
  • 1 (D.C.)
  • 2 a
  • 3 Intelligent Synthetic Biology Center, Daejeon 34141, Korea
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Feb 02, 2020
Volume
21
Issue
3
Identifiers
DOI: 10.3390/ijms21030990
PMID: 32024292
PMCID: PMC7037952
Source
PubMed Central
Keywords
License
Green

Abstract

A large proportion of the recombinant proteins manufactured today rely on microbe-based expression systems owing to their relatively simple and cost-effective production schemes. However, several issues in microbial protein expression, including formation of insoluble aggregates, low protein yield, and cell death are still highly recursive and tricky to optimize. These obstacles are usually rooted in the metabolic capacity of the expression host, limitation of cellular translational machineries, or genetic instability. To this end, several microbial strains having precisely designed genomes have been suggested as a way around the recurrent problems in recombinant protein expression. Already, a growing number of prokaryotic chassis strains have been genome-streamlined to attain superior cellular fitness, recombinant protein yield, and stability of the exogenous expression pathways. In this review, we outline challenges associated with heterologous protein expression, some examples of microbial chassis engineered for the production of recombinant proteins, and emerging tools to optimize the expression of heterologous proteins. In particular, we discuss the synthetic biology approaches to design and build and test genome-reduced microbial chassis that carry desirable characteristics for heterologous protein expression.

Report this publication

Statistics

Seen <100 times